Versatility: the same gel can be used for western blotting, standard staining methods, and mass spectrometry analysis of proteins.Greater transfer efficiency: proteins can be transferred onto PVDF or nitrocellulose membranes in as little as 3 minutes with the Trans-Blot Turbo system.Other key benefits of Mini-PROTEAN TGX Stain-Free precast gels include: Researchers can produce high-quality images using the Gel Doc EZ or ChemiDoc MP imaging systems in as little as 2.5 minutes.Ī UV-induced reaction between the gel’s trihalo compounds and the tryptophan residues of the proteins results in fluorescence that is easily detected in gels and on PVDF membranes after transfer. We routinely use slightly less sample and far fewer reagents and continue to see excellent gel-to-gel consistency and significantly faster experimental throughput without a considerable overall cost increase per result.” “It’s critical for experiments to be both time- and cost-effective,” said Dr David Warren Pryce, PhD, principal investigator, North West Cancer Research Fund Institute, Bangor University, Wales UK.ĭr Pryce continued, “Since using Bio-Rad’s Mini-PROTEAN TGX Stain-Free precast gels and buffers and the Trans-Blot Turbo transfer system, I have found that analysis that once took - at minimum - 1.5 hours to prepare and perform can now be completed in 20 minutes. This eliminates the need for staining, thus saving time while increasing the rate of success for western blotting experiments. They can then visualize and verify protein transfer directly on the membrane with stain-free imagers such as Bio-Rad’s Gel Doc™ EZ or ChemiDoc™ MP imagers. Researchers can transfer proteins from the Mini-PROTEAN TGX Stain-Free precast gels onto membranes in three minutes using the Trans-Blot® Turbo™ transfer system. The V3 Western Workflow increases speed at every step - from protein separation to transfer verification. When researchers use the Mini-PROTEAN TGX Stain-Free precast gels as part of Bio-Rad’s V3 Western Workflow™ they can achieve complete protein separation, gel imaging, and data analysis in 20 minutes. Polyacrylamide gel staining methods that follow Coomassie protocols can take up to two hours to complete. The gels include a unique trihalo compound that allows rapid fluorescent detection of proteins in gels or on membranes without the need for staining. has announced that it has launched its Mini-PROTEAN TGX (Tris-Glycine eXtended) Stain-Free precast gels, providing researchers with the fastest separation and imaging capabilities available for Laemmli PAGE. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting, and may be visualized in gels or multiple times on PVDF membranes.Ĭassette opening levers ( 4560000) are sold separately.Bio-Rad Laboratories, Inc. Mini-PROTEAN TGX Stain-Free Gels contain trihalo compounds, which in the presence of UV-light react with tryptophan residues producing fluorescence. Proteins retain native conformation and activity in the absence SDS.Laemmli-like separation in the presence of SDS.Can be used for denaturing electrophoresis with standard running buffer containing SDS.Stain-free imaging of gels and blots in 1 min.There is a wide range of Mini-PROTEAN TGX Stain-Free Precast Protein Gels, including other gels with 12 wells, in both single percentages and gradients. Run up to 4 Mini-PROTEAN TGX Stain-Free Protein Gels in 35 min using the Mini-PROTEAN Tetra Cell. TGX Stain-Free Gels can be used for both standard denaturing protein separations as well as native electrophoresis. Long-life TGX, Tris-Glycine eXtended, Stain-Free Gels have a novel formulation, which allows rapid fluorescent detection of proteins with Bio-Rad’s stain-free enabled imaging systems. Use these Mini-PROTEAN TGX Stain-Free Protein Gels for separation of polypeptides 2–400 kD.
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